![]() ![]() ![]() Ca 2+ indicators) into living cells by either dye loading or gene expression 12, 13. This method delivers fluorescent molecules (i.e. Ca 2+ imaging), fluorescence imaging has been broadly used for its outstanding sensitivity and specificity 9– 11. To monitor intracellular calcium dynamics in real time (i.e. Intracellular calcium concentration () plays important roles in the regulation of a variety of cellular functions 1, 2, such as muscle contraction 3, 4, neurotransmitter release 5, 6, and gene expression 7, 8. Our results suggest the possible use of the spectrally filtered array towards a miniaturized on-chip fluorescence imaging device, which may open up new opportunities in tissue-level pharmaceutical screening and fundamental studies on cell networks. Importantly, our array-level data qualitatively captured the static fluorescence image of the cells and the intracellular Ca 2+ dynamics, both of which are correlated with the microscope-collected data. ![]() Employing fluorescence microscopy as the reference, we demonstrate that our array can conduct on-chip Ca 2+ imaging in C2C12 cells that were chemically triggered to increase their intracellular Ca 2+ levels. ![]() Coated with a spectral filter layer that has a high extinction ratio (>10 3), our array features high wavelength selectivity (>10 2), high linearity ( R 2 > 0.98), and low detection limit (45.1 μW 640/30 nm light). In this work, we report a 100%-yield, spectrally filtered passive Si photodiode array designed for on-chip fluorescence imaging of intracellular Ca 2+ dynamics. However, it is challenging to integrate a spectral filter onto such devices (to block the excitation light) that has similar performance to the state-of-the-art emission filters used in fluorescence microscopes. On-chip fluorescence imaging devices are recognized for their miniaturized and implantable nature that can benefit the study of intracellular dynamics at a variety of settings. ![]()
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